Antioxidant and Free Radical Scavenging Activity of Trigonella foenum-graecum L, Murraya koenigii , Coriandrum sativum and Centella asiatica

Authors

  • Sanghamitra Dutta Bengal Institute of Technology
  • Debanita Roy Bengal Institute of Technology
  • Archisman De Bengal Institute of Technology
  • Camellia Dutta Bengal Institute of Technology
  • Swagata Bhattacharya Bengal Institute of Technology

DOI:

https://doi.org/10.24925/turjaf.v4i4.255-261.520

Keywords:

culinary herbs, leaf extract, antioxidants, free radical scavenger

Abstract

Antioxidants are naturally occurring substances that combat oxidative damage in biological entities. An antioxidant achieves this by slowing or preventing the oxidation process that can damage cells in the body. It does this by getting oxidized itself in place of the cells. The aim of the present study was to evaluate the in vitro antioxidant and free radical scavenging activities of aqueous and 95% methanol leaf extracts of four herbs viz. Trigonella foenum-graecum L, Murraya koenigii, Coriandrum sativum and Centella asiatica which have frequent use in Indian cuisine. Both aqueous and 95% methanol leaf extracts have shown significant amount reducing power. Both aqueous and 95% methanol leaf extracts of Coriandrum sativum had significant DPPH radical scavenging activity with IC50 value of 0.21± 0.3 mg/L and 0.176 ± 0.008 mg/L respectively. The aqueous leaf extract of Trigonella foenum-graecum L showed low scavenging activity. Among all the leaf extracts, the aqueous leaf extract of Centella asiatica has exhibited significantly high NO radical scavenging activity (80%) with IC50 value of 0.11 ± 0.17 mg/L. The aqueous leaf extracts of the samples have showed significantly high superoxide radical scavenging activity. The activity was maximum for the aqueous leaf extract of Centella asiatica, IC50 value is 4.36 ± 0.41 mg/L. anti lipid peroxide activities were very high ( 90 %) for aqueous leaf extracts of Coriandrum sativum (IC50 = 0.064 ± 0.85 mg/L) and Centella asiatica (IC50 = 0.066 ± 0.9mg/L) at a concentration of 0.16 mg/L. The aqueous leaf extracts of the samples were found to contain large amounts of flavonoids and phenolic compounds and exhibited high antioxidant and free radical scavenging activities. These in vitro assays indicate that these plant extracts are significant source of natural antioxidants which might be helpful in preventing the progress of various oxidative stresses.

Author Biographies

Sanghamitra Dutta, Bengal Institute of Technology

Department of Biotechnology Assistant Professor

Debanita Roy, Bengal Institute of Technology

Department of Biotechnology, Student

Archisman De, Bengal Institute of Technology

Department of Biotechnology, Student

Camellia Dutta, Bengal Institute of Technology

Department of Biotechnology, Student

Swagata Bhattacharya, Bengal Institute of Technology

Department of Biotechnology, Student

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Published

13.04.2016

How to Cite

Dutta, S., Roy, D., De, A., Dutta, C., & Bhattacharya, S. (2016). Antioxidant and Free Radical Scavenging Activity of Trigonella foenum-graecum L, Murraya koenigii , Coriandrum sativum and Centella asiatica. Turkish Journal of Agriculture - Food Science and Technology, 4(4), 255–261. https://doi.org/10.24925/turjaf.v4i4.255-261.520

Issue

Section

Food Technologies