In vitro Multiple Shoot Regeneration from Petunia hybrida

Rebaz Rasul Habas; Musa Turker; Fethi Ahmet Ozdemir

doi:10.24925/turjaf.v7i10.1554-1560.2570

Authors

Rebaz Rasul Habas Department of Molecular Biology and Genetics, Institute of Natural and Applied Science, Van Yüzüncü Yıl Universtiy, 65080 Van http://orcid.org/0000-0003-4022-3287
Musa Turker Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yıldız Technical University, 34210 Istanbul http://orcid.org/0000-0003-3195-1119
Fethi Ahmet Ozdemir Department of Molecular Biology and Genetics, Faculty of Science and Art, Bingol University, 12000 Bingol http://orcid.org/0000-0001-7215-9692

DOI:

https://doi.org/10.24925/turjaf.v7i10.1554-1560.2570

Keywords:

Petunia hybrida, In vitro, Multiple shoot, Regeneration, Gibberellic acid

Abstract

An efficient plant regeneration protocol was developed from in vitro germinated seeds of Petunia hybrida an ornamentally important plant in the family Solanaceae. Shoot tip and node explants of Petunia hybrida were cultured on MS basal medium supplemented with different concentrations and combinations of Benzyl amino purine (BAP), 1-Naphthaleneacetic acid (NAA), Indole-3-butyric acid (IBA) and Gibberellic acid (GA3). The highest shoot length was obtained from MS medium supplemented with 1 mg/l BAP + 1 mg/l NAA. The highest shoot number (3 shoots/explant) were obtained from MS medium supplemented with 0.6 mg/l BAP + 0.5 mg/l IBA. The isolated shoots were transferred to MS basal medium supplemented with different concentrations of GA3 ranging from 0.05, 0.2, 0.5 and 1 mg/l for shoot elongation. The highest shoot length (5.75 cm) was recorded from the MS medium supplemented with 0.2 mg/l GA3 +0.2 mg/l BAP. Rooting of regenerated shoots were achieved on MS medium supplemented with 0.1-1 mg/1 IBA and NAA. The regenerated shoots with well developed roots were successfully acclimatized and established in pots containing sterilized peat moss and grown under laboratory conditions with 70% survival rates.